Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Frontiers Glutathione deficiency in the pathogenesis of SARS CoV 2 infection and its effects upon the host immune response in severe COVID 19 disease Frontiers Deuterium trafficking, mitochondrial dysfunction, copper homeostasis, and neurodegenerative disease How Keeping Glutathione Levels Up Can Benefit Your Health & Your Vitamin D Status GrassrootsHealth